mScarlet3

Reliable CRISPR/Cas9 genome engineering in Caenorhabditis elegans using a single efficient sgRNA and an easily recognizable phenotype

Update 2017-07-18


 126 Labs and counting!  wrmScarlet is freely available for all labs who want it. Visit the following page for the page dedicated to this paper.

To efficiently and freely distribute the wrmScarlet sequence to the community, we have decided to list here all the labs that we have sent the sequence to. Please contact us or anyone on the list to get your copy of the plasmid.
More information here

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21th International Worm Meeting

Great Meeting!

Posters, check! Talk, check! 30 wrmScarlet vectors gone in 3 min, check! 70+ requests to be sent out in the coming days, pending...

And to top it off, Sonia wins the Dr. Matthew J. Buechner Tie Award for service to the community!

July 6, 2017

"Genes to Genomes" announces #Worm17 GSA Poster Award Winners

2017_LA

  medaille-bronze

Institut NeuroMyoGène


We are now part of the Institut NeuroMyoGène.

The INMG is a novel institute dedicated to the study of the nervous and muscular systems. It was created in 2016 in Lyon at the heart of a dense hospital network and of one of the biggest medical universities in France.

2017-03-02_G3

2017-03-02 - Finally!


Our paper has just been accepted at G3: GENES, GENOMES, GENETICS!

Congratulations to the whole team! It was a great collective effort.

 We want to understand how cellular physiology is controled by basic molecular and cellular pathways regulating the biology of potassium-selective ion channels.

Two-pore domain potassium channels (K2P) play a central role in the control of cellular excitability and the regulation of the cell's electrical membrane potential. K2Ps have been widely conserved throughout evolution. They are polymodal ion channels that are subjected to extensive regulation by a diverse set of physical (pH, temperature, mechanical force) and biological signals (lipids, G-protein coupled receptor pathways). They are broadly expressed in excitable and non-excitable cells, and have in turn been implicated in a large spectrum of physiopathological processes, ranging from the regulation of neuronal excitability, respiratory and cardiac function to the control of cell volume, hormone secretion and cell proliferation. Recently, loss- and gain-of-function mutations in K2P channels have been directly linked to human pathologies (Birk Barel syndrome, familial migraine with aura, cardiac conduction disorder).

In contrast to many other ion channel families, comparatively little is known about the molecular and cellular processes that regulate different aspects of the cell biology of K2P channels. For instance we know only of very few factors that specifically regulate the expression, the activity and the localisation of K2P channels at the cell surface. Therefore the central question addressed by our team is: How is the number of active potassium leak channels present at the cell surface controlled in vivo?

To identify novel genes and conserved cellular processes that regulate the biology of K2P channels in vivo we take advantage of the powerful genetic tools available in the model nematode Caenorhabditis elegans. We use the full array of techniques available in C. elegans including genetics, live imaging, electrophysiology and state-of-the-art CRISPR/Cas9 genome engineering and next-generation DNA sequencing. These studies will provide new leads to understand the cellular pathways that control K2P function in other organisms.

  medaille-bronze

CNRS Bronze Medal


Thomas has received his Bronze medal during the official CNRS ceremony on December 4th, 2014

"La Médaille de bronze récompense le premier travail d'un chercheur, qui fait de lui un spécialiste de talent dans son domaine. Cette récompense représente un encouragement du CNRS à poursuivre des recherches bien engagées et déjà fécondes."

Press releases: CNRSUniversité de LyonFaculté des SciencesERC

  logo-ERC

Interested?


We are always looking for highly motivated Post-docs, Masters and PhD students to participate in our ERC Starting Grant-funded projects.
Please consult this page before applying.

 

Our Projects

We endeavor to understand how basic molecular and cellular pathways regulating the biology of potassium-selective ion channels control cellular physiology.

Lab Members

Funding & Support

Our research is funded by the European Research Council (ERC Stg 2013) and the Fondation Fyssen

Our Publications

A generally up-to-date list of our publications and meeting abstracts.

The Newsstand

An ever growing list of papers that stand out.

Tools

References to tools that might be of interest.

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Welcome to Lyon. The capital of the Gauls (Lugdunum) is famous for its gastronomy and architecture.

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