LOCUS       pSEM87_twk-18_wrmScarlet  6637 bp ds-DNA     circular     04-JAN-2017
DEFINITION  .
COMMENT     [Boulin Lab, 2017] contains wrmScarlet cDNA sequence (no introns, no stop). 
FEATURES             Location/Qualifiers
     misc_feature    3308..4003
                     /label="wrmScarlet"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     exon            4817..4944
                     /label="twk-18|Ex2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3308..4003
                     /label="wrmScarlet"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(759..777)
                     /label="oBP225"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1235..3307
                     /label="LHR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4004..5996
                     /label="RHR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(186..845)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            5795..5833
                     /label="twk-18|Ex5"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            4993..5194
                     /label="twk-18|Ex3"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     1189..1208
                     /label="T7"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     exon            5562..5749
                     /label="twk-18|Ex4"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(6043..88)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     exon            4004..4061
                     /label="twk-18|Ex1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
       61 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      121 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
      181 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
      241 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
      301 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
      361 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
      421 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
      481 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
      541 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
      601 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
      661 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
      721 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
      781 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
      841 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
      901 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
      961 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1021 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1081 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1141 gttccgcgca catttccccg aaaagtgcca cctgacaaac ttggtaccta atacgactca
     1201 ctatagggGG CGCGCCtcga CCTGCAGGtc gaGCatggga attggtgcat tttcaaaagc
     1261 aagtgagatt ttttgcaata actttttgaa cagctcaaag aagatacaag ctttttcatc
     1321 atttgaagtt tgacatcgtc ttcttagttt tcatgcaaat cttagggctg atcattctaa
     1381 ctagattttt gaagtttttt taattgttct ttgaaccgat gttaaacaca taatttagtt
     1441 ttctgtacag ttatttagtt cccctctagt ataacaaaat ctctctctct ctctctctga
     1501 actaaaacca attagtcagt tatctttcat ttcgtttgtt ttttatcttt ttgtgttaca
     1561 atcctgagaa ttttcctccc ctcccaccca tatcattgcc gtttcatttc aagggcccgc
     1621 tcatcaaatt gttgtgacga ttgactccaa agacctcacc gatctatctt ctcttttttt
     1681 cgctttgcat ttttttaccc gcaccgatgc ctaccaatat caaaatatcc aaagtgtcag
     1741 agtcggatgg cacgcggcag aggtctgaca caaaatcgaa caattggcac cctgccgcca
     1801 ttgaaaaacg agtgacagaa aaatgaaaag gacaactgaa cagattgaag aagctgcaaa
     1861 aagtgtatta tatccaatga gtgtgcctgt gtttgtgagt gtcacatctt tcctgctccg
     1921 tatgctccac attctccttc ttttgtcaac ctctcccgcg tcgcctcccg cttctcaact
     1981 tttgcccccc gggggcgcta tcagcaaatc cgtccgtgtg ttattctccc gaacgaactt
     2041 ttcagagagc atatgggctt tcaaagtctt tttggtttac aattcatttt ttcaagtaga
     2101 tgatactttc atcgtatttc atgaagacct tgaacgactg tgttttttaa catctagtat
     2161 tccattgtaa aaataagttt ctcaaaaagc cacaatatat tgaaataagt ttcaacattt
     2221 tggtatgcca tccaaccacc cataaagtta atgtatctaa acaatgatct tctgaaaaag
     2281 gtttcccata ctaatttgca ttttgaatgg gtcccttaca atttggcttt aaggctaaca
     2341 ttcaaaacat tgaataaaga cattttaaat ttactctaag aaatccaaaa cgactaacct
     2401 cttaaagttc aatctaaaat ttgttcaatt tattttttaa ctacgttcaa gtcctgttaa
     2461 attgttttaa cagctgtgct tggaaggctt ttgaaggttt tgcggtattg agaatcaatt
     2521 atgcattttc gacgctattt tctttcgcgt ttaactttaa actttaaaat tgtttttttt
     2581 ttaaatttca acgtaactca ataaattttt taaagaaaca tgtaaaatga ttttcagatc
     2641 gtccatccac tctcaaacgc attgaaaaac attaaaagtc tttaaagaca gattttggaa
     2701 acattctaca aaactcgaaa atagtgaaac aatgcacaaa aaaaatttca gatgggcgtt
     2761 gtgccactaa acattttgct gtggcatatc ctcattgtgg gcagtctcca ataaaaaatt
     2821 ctcgtgaaaa atgtctgtag taaattgaaa gatgattgaa gattttaaag catttcaact
     2881 tctggtattt ctactcttgg agtagtatta taaaagttgt gactgtggtg gtttcagaaa
     2941 aaaaacctca caactaatat gaaagttttg atctttaata acatgttgaa attcccttat
     3001 ctttgcattc tgggcgaatt tatgattgcc aataacctag actccgattg cccccccccc
     3061 ctttccaacc cctctcatcc ttttctctca tcaactttag ctatcagata agctttccct
     3121 ccaagcaatc ccactcactt tgctccatat caattttgca catcttcttc gttttttgtc
     3181 gtttttgaaa aaaaacccat tctttaaaga ccaaaaatct cacctaaacc acacaagttt
     3241 cataaaaagt agtattcagg tagtacacga aaggtaggag gcaagcgggt gacaaaaaca
     3301 accggaaATG GTCAGCAAGG GAGAGGCAGT TATCAAGGAG TTCATGCGTT TCAAGGTCCA
     3361 CATGGAGGGA TCCATGAACG GACACGAGTT CGAGATCGAG GGAGAGGGAG AGGGACGTCC
     3421 ATACGAGGGA ACCCAAACCG CCAAGCTCAA GGTCACCAAG GGAGGACCAC TCCCATTCTC
     3481 CTGGGACATC CTCTCCCCAC AATTCATGTA CGGATCCCGT GCCTTCACCA AGCACCCAGC
     3541 CGACATCCCA GACTACTACA AGCAATCCTT CCCAGAGGGA TTCAAGTGGG AGCGTGTCAT
     3601 GAACTTCGAG GACGGAGGAG CCGTCACCGT CACCCAAGAC ACCTCCCTCG AGGACGGAAC
     3661 CCTCATCTAC AAGGTCAAGC TCCGTGGAAC CAACTTCCCA CCAGACGGAC CAGTCATGCA
     3721 AAAGAAGACC ATGGGATGGG AGGCCTCCAC CGAGCGTCTC TACCCAGAGG ACGGAGTCCT
     3781 CAAGGGAGAC ATCAAGATGG CCCTCCGTCT CAAGGACGGA GGACGTTACC TCGCCGACTT
     3841 CAAGACCACC TACAAGGCCA AGAAGCCAGT CCAAATGCCA GGAGCCTACA ACGTCGACCG
     3901 TAAGCTCGAC ATCACCTCCC ACAACGAGGA CTACACCGTC GTCGAGCAAT ACGAGCGTTC
     3961 CGAGGGACGT CACTCCACCG GAGGAATGGA CGAGCTCTAC AAGgcgattg ttgcgcaagg
     4021 agtttctacg attttgacga cgtttcagaa aacgttcaaa ggtatgtcat atgtactttt
     4081 tgaaaggaga accatgtgat gttttattga tattcattat ttaaacgttg ctttaaatat
     4141 aatttctttc gaaaaatgat tttctttatt cggatgatac gtaccgcaat ttcaagtaag
     4201 ttttgattta aaataacaaa gatgtttctg aatgaaaatc gactatgaaa agtaattatt
     4261 tcttgaaatg attgtgaaac attaatctga aaaataaaat catattcttc agatatgtag
     4321 attcaaatcc cttatctaat acaggaactc aataaatact gtgaggagtt tgtaaacaaa
     4381 taccctaaac ttatgaataa gaatatttgt aacggatacc atattattgc tatatgtaga
     4441 taggtaggaa tacctactta ctgctcattt gacgtatttc gaagaaaaat caattcgatt
     4501 tctcatcaat attttcctta aaacgtttta gctgtttctc gctatgtata tgtaggtgtt
     4561 tttcatgatt gttacatact tgttgagcat atttgcaatt ttaaacataa gtatatattt
     4621 tttcaattca caatcaatga accgccgtta ttctgaatca gaatagaaca taaatttaat
     4681 tacgaactag tctccctcat tcttttataa tcttcgttca ctcacaatta cactatgttt
     4741 tgaccgcaca aaatttaacg tagtaatatc acaacaggat ctagtctccg aaatgattat
     4801 cttcaatctg tttcaggatt actcccattg ataattcttg tggcttacac tctattggga
     4861 gcatggatct tctggatgat cgaaggagaa aacgaacgag agatgctcat tgagcaacaa
     4921 aaagaaaggg atgaactcat ccgggtatcg aatatttaga tagaatgaat gattatgaaa
     4981 agttgttttt agcgaactgt ctataaaatt aatcaattgc aaatcaaaag acagagaaga
     5041 ttaatgacgg ccgaggaaga atacaacaga actgcaaaag tgttgactac ctttcaagaa
     5101 actttgggaa ttgtaccagc tgatatggac aaggatattc attggacatt cttgggatca
     5161 atcttctatt gcatgacagt ctatacaact attggtaaga aacacaacaa attggtattg
     5221 aatttttata tttgaatgtt caatttttag ttgaagagta atgttttcct tgcggcgctt
     5281 aagtatttga ataacagagt aaaattttcc aaaaactttt tgaaatgttt tacttacagt
     5341 caaaaagtgt caattttttg tattcacgat gttctattat tgtcaaggaa aataaataga
     5401 ctgctattat aaaaaatgag gaccttgaat attgctagga aaaaatatta acatccagat
     5461 agtaacgaat cacattcgtt tcaatttatt actattgacc gtagttgcat cttgttcata
     5521 aattttagaa accaatatac aattagtatt ttgcatttca ggttacggaa acattgttcc
     5581 tggaactgga tggggtcgtt ttgctactat cctttacgca ttcattggaa ttccactcac
     5641 agtgctcagt ctttactgtc ttggtagtct ttttgccaag ggatgcaaaa tgttgtggag
     5701 attttttttg aagtccacca gggttgtgag caaagatttg tcaaataagg tgggaaattt
     5761 tgaatggcaa aacaactata agaatatttt ttagatttct gaagctgccg ataatattga
     5821 agaaggaaca acggtgagat aatgtttcca aatatttctc aaaattaatt tgttcaggca
     5881 attactccat cagccgagaa aacagaaaat aacgatgatg acttgttgtc tttccccatc
     5941 tccggcctct tgcttattac cgtcatctgg gtcattttct gtgcggtctt gttcacctgc
     6001 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6061 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6481 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6601 tttttttgtt tgcaagcagc agattacgcg cagaaaa
//