LOCUS       pPT2                    3448 bp ds-DNA     circular     06-NOV-2014
DEFINITION  .
COMMENT     sgRNA expression vector; generated by Philippe Tardy - Boulin Lab - 2014-2017                    
COMMENT     CNRS - *www.excitingworms.eu*                                      
FEATURES             Location/Qualifiers
     promoter        305..323
                     /label="tac promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     305..324
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        372..871
                     /label="U6 pol III promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    884..966
                     /label="sgRNA scaffold"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(1619..2301)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(1237..1260)
                     /label="pJET_rev AAGAACATCGATTTTCCATGGCAG"
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_fwdcolor=cyan
     CDS             complement(2399..3058)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     310..332
                     /label="pJET_fwd CGACTCACTATAGGGAGAGCGGC"
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_fwdcolor=cyan
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tCTCCAAGAA CTCGTACAAA AATGCTCTGA AGTAGGTCTC GAGATCAATA
      421 CTGGGAAGAC GAAAGTCTTG CGAAACCGAT TCGCTGACCC CAGTAAAGTC TACTTCGGTA
      481 GCCCTTCCCC CACCACCCAG CTCGACGACG TCGACGAGTA CATCTACCTC GGTCGTCAAA
      541 TCAACGCCCA AAACAACTTG ATGCCGGAAA TCCACCGAAG ACGTCGAGCA GCCTGGGCTG
      601 CATTCAATGG AATCAAGAAT GCCACCGACT CCATCACCGA CAAGAAGATT CGTGCGAATC
      661 TGTTCGACTC AATTGTCCTT CCAGCGCTCA CCTACGTTTC AGAAGCCTGG ACATTCACCA
      721 AAGCTCTATC CGAACGAGTA CGAATCACAC ATGCCTCCCC CACAAATTAT TGATCTACAT
      781 CCCCATTTGG TATAGTGTCT TACAGGACTC TCAATATGTC AGCTGCATGA TAAATGGTCC
      841 CTATATAATT GAATTGCAAA TCTAAATGTT TaaacTTTac cagGTTTTAG AGCTAGAAAT
      901 AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCT
      961 TTTTTTCAAT AATATTCTAG ATATCCCTTT TTGAAACTAA AATGAAATAC TCTAAATTTT
     1021 TGAGTTCAAT GTTTCAACTA ATCTTGGTTA AAGGTAAATT CCCGGAGGGA CGATTTTAAA
     1081 GTTGCCAGAG TTGATTAAAT TGGTCCAAAA AATTTTATTA GGAAACTCGA AAAAAAATTG
     1141 ATTTTTTTTG ACAATATTCC AAAGAAAATT ACAAAATCTC GACGCCAAAC ATAGTCGGCT
     1201 GTGatctttc tagaagatct cctacaatat tctcagctgc catggaaaat cgatgttctt
     1261 cttttattct ctcaagattt tcaggctgta tattaaaact tatattaaga actatgctaa
     1321 ccacctcatc aggaaccgtt gtaggtggcg tgggttttct tggcaatcga ctctcatgaa
     1381 aactacgagc taaatattca atatgttcct cttgaccaac tttattctgc attttttttg
     1441 aacgaggttt agagcaagct gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1501 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1561 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1621 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1681 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1741 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1801 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1861 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1921 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1981 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2041 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     2101 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2161 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2221 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2281 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2341 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2401 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2461 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2521 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2581 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2641 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2701 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2761 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2821 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2881 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2941 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3001 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3061 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3121 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3181 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3241 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3301 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3361 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3421 cctataaaaa taggcgtatc acgaggcc
//