LOCUS pMD8_dpy-10_sgRNA 3456 bp ds-DNA circular 09-FEB-2016 DEFINITION [Boulin Lab] sgRNA expression vector targeting the d10 site. KEYWORDS "sgRNA" COMMENT [Boulin Lab, 2017] sgRNA expression vector targeting the d10 site. FEATURES Location/Qualifiers primer_bind complement(1245..1268) /label="pJET_rev AAGAACATCGATTTTCCATGGCAG" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 promoter 372..871 /label="U6 pol III promoter" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 872..891 /label="CRpMD8" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 305..324 /label="T7" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 310..332 /label="pJET_fwd CGACTCACTATAGGGAGAGCGGC" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 892..974 /label="sgRNA scaffold" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 CDS complement(2407..3066) /label="AmpR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin complement(1627..2309) /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 ORIGIN 1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc 61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc 121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact 181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt 241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc 301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt 361 ttcagcaaga tCTCCAAGAA CTCGTACAAA AATGCTCTGA AGTAGGTCTC GAGATCAATA 421 CTGGGAAGAC GAAAGTCTTG CGAAACCGAT TCGCTGACCC CAGTAAAGTC TACTTCGGTA 481 GCCCTTCCCC CACCACCCAG CTCGACGACG TCGACGAGTA CATCTACCTC GGTCGTCAAA 541 TCAACGCCCA AAACAACTTG ATGCCGGAAA TCCACCGAAG ACGTCGAGCA GCCTGGGCTG 601 CATTCAATGG AATCAAGAAT GCCACCGACT CCATCACCGA CAAGAAGATT CGTGCGAATC 661 TGTTCGACTC AATTGTCCTT CCAGCGCTCA CCTACGTTTC AGAAGCCTGG ACATTCACCA 721 AAGCTCTATC CGAACGAGTA CGAATCACAC ATGCCTCCCC CACAAATTAT TGATCTACAT 781 CCCCATTTGG TATAGTGTCT TACAGGACTC TCAATATGTC AGCTGCATGA TAAATGGTCC 841 CTATATAATT GAATTGCAAA TCTAAATGTT TgCTACCATA GGCACCACGA GGTTTTAGAG 901 CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG 961 TCGGTGCTTT TTTTCAATAA TATTCTAGAT ATCCCTTTTT GAAACTAAAA TGAAATACTC 1021 TAAATTTTTG AGTTCAATGT TTCAACTAAT CTTGGTTAAA GGTAAATTCC CGGAGGGACG 1081 ATTTTAAAGT TGCCAGAGTT GATTAAATTG GTCCAAAAAA TTTTATTAGG AAACTCGAAA 1141 AAAAATTGAT TTTTTTTGAC AATATTCCAA AGAAAATTAC AAAATCTCGA CGCCAAACAT 1201 AGTCGGCTGT Gatctttcta gaagatctcc tacaatattc tcagctgcca tggaaaatcg 1261 atgttcttct tttattctct caagattttc aggctgtata ttaaaactta tattaagaac 1321 tatgctaacc acctcatcag gaaccgttgt aggtggcgtg ggttttcttg gcaatcgact 1381 ctcatgaaaa ctacgagcta aatattcaat atgttcctct tgaccaactt tattctgcat 1441 tttttttgaa cgaggtttag agcaagctgc ttcctcgctc actgactcgc tgcgctcggt 1501 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 1561 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 1621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 1681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 1741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 1801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 1861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 1921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 1981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 2041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 2101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 2161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 2221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 2281 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 2341 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 2401 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 2461 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 2521 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 2581 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 2641 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 2701 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 2761 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 2821 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 2881 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 2941 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 3001 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 3061 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 3121 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 3181 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 3241 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 3301 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 3361 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 3421 gacattaacc tataaaaata ggcgtatcac gaggcc //